Some genetic diseases cannot be diagnosed by changes in restrictionsites.
Some of these can be detected by allele-specific oligonucleotideprobes.
These are

copies of the gene with an altered sequence so that a restriction site is inserted
 
mutagenized copies of a gene
 
short sequences that will hybridize only to a specific base sequence
 
PCR-amplified variable numbers of tandem repeats (VNTRs)
 

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